In this study, cecal ligation and puncture were performed in 75 Sprgue-Dawley rats. Peritoneal lavage was performed using 10 ml of povidone-iodine as 1% concentration in group A, while the same volume of 50% of honey diluted in distilled water was used in group B. Group C was used as a control. The overall mortality was 32% in group A (8/25), 12% in group B (3/25) and 60% in group C (15/25). Tissue toxicity was found to outweigh bactericidal effects. Honey was tested for its broad-spectrum antibacterial activity without adverse effects. So, we concluded that honey might be used as a peritoneal lavage solution with no side effects.