In the present study, serological diagnosis of brucellosis, Johne’s Disease (JD) and infectious bovine rhinotracheitis (IBR) was carried out in the states of Gujarat and Andhra Pradesh of India. Apparent prevalence of brucellosis was 7.57 % (true prevalence, 5.66%) in Gujarat and 12.27 % (true prevalence, 10.60%) in Andhra Pradesh. For JD apparent prevalence in Gujarat and Andhra Pradesh was 13.39% (true prevalence, 15.68%) and 16.26% (true prevalence, 19.31%) respectively. Apparent prevalence of IBR was found to be 23.94% (true prevalence, 18.20%) and 26.49% (true prevalence, 21.03%) in Gujarat and Andhra Pradesh respectively. The results of the study revealed that these diseases are highly prevalent in both the states; hence, there is an urgent need for adopting suitable control measures.