Understanding how organisms respond to fluctuating nutritional stimuli, through cell cycle dynamic changes, and reproducibly reach distinct and balanced cell cycle parameters in a wide range of steady-state growth conditions is important with wider implications across all domains of life. Bacterial growth rates vary according to the availability of metabolic nutrients and are linked to DNA synthesis by modulating either replication initiation frequency or speed of DNA synthesis. Despite this intimate link between growth and replication the molecular details that underpin this link at the regulatory and functional levels remain largely unknown.
A genetic link between the lower half of glycolysis/gluconeogenesis and DNA replication in B. subtilis has been discovered. We hypothesize that there is a sophisticated regulatory system that adjusts the activity of the replication machinery in response to the cell’s metabolism. Signals originating from the Central Carbon Metabolism core and sensed by DnaA, DnaE, DnaC and DnaG to control initiation and elongation of replication, thereby gating replication in the cell cycle. These signals regulate structural/functional changes in key replication enzymes; the replicative helicase DnaC, the primase DnaG and the polymerase DnaE, as well as the replication initiator protein DnaA.
This project aims to uncover the molecular details of the mechanism that links metabolism to DNA replication.
The incoming Research Fellow should hold a PhD in a relevant life sciences area and have experience in molecular microbiology, protein biochemistry, bacterial genetics, molecular biology. Direct experience in biochemical assays with DNA replication and/or metabolic proteins would be a distinct advantage.
The appointed postdoctoral research fellow is expected to assume a leading role within the lab, supervise PhD students and look after the running of the lab on a day-to-day basis.
The successful applicant will be expected to start work anytime between 01 October 2018 and 01 December 2018, to be agreed. This full time post will be offers on a fixed term contract for a period of 3 years.
Closing DateFriday, 13th July 2018
Short-listed applicants will be invited to interview the week commencing 06/08/2018.
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Informal enquiries may be addressed to Professor Panos Soultanas, Email: Panos.email@example.com
Please note that applications sent directly to this email address will not be accepted.
The University of Nottingham is an equal opportunities employer and welcomes applications from all sections of the community.