Packed Cell Volume (PCV)

Last Updated on October 22, 2021 by admin DefinitionPacked cells volume (PCV) or Hematocrit, is defined as the percent of blood occupied by RBCs, or simply you can define PCV as the percent of RBCS in whole blood. To measure PCV, the RBCS must be packed together in one layer, which can be achieved by … Read more

Components of blood

Last Updated on December 7, 2018 by admin Blood is composed of two parts: fluid part and cellular part. The fluid part is called the plasma, in which the cells, nutrients and blood gases circulate. The cellular part composed of red blood cells (RBCs), white blood cells (WBCs) or leucocytes and platelets. The fluid part … Read more

Serum enzymes

Last Updated on December 7, 2018 by admin Enzymes are specialized and very specific proteins, which catalyze various chemical reactions. Research work and application of clinical enzymology in medicine have been expanded rapidly, it plays an important role in diagnosis, prognosis and evaluation of different diseases and metabolic disorders (Bogin and Sommer, 1976). Most of … Read more

Plasma proteins

Last Updated on December 7, 2018 by admin Measured serum proteins include total proteins, albumin and globulin. The latter can be separated by electrophoresis into α-globulin, β-globulin and γ-globulin. Total proteins level measured in plasma is higher than that measured in serum, this because another protein present in plasma, which is fibrinogen. Commercially supplied kits … Read more

Morphological examination of erythrocytes

Last Updated on December 7, 2018 by admin All parameters of the erythrocyte picture can be determined using a blood cell counter, except assessing the morphological appearance of the RBCs, which must be evaluated using blood smear. This means, that both electronic blood cell count and blood smear are required to perform the erythrocyte picture. … Read more

Basics of hematological analysis (Complete blood count)

Last Updated on December 7, 2018 by admin Prof. Mahmoud Rushdi (Assiut University-Egypt) Hematological analysis is concerned with the analysis of the cellular component of blood including tests that used for evaluation of erythrocytes, leucocytes and platelets. Blood sample for complete blood count (CBC) must be collected on EDTA as anticoagulant. Evaluation of the erythrocytes … Read more

Anticoagulants

Last Updated on December 7, 2018 by admin Anticoagulants are chemical substances that can prevent blood coagulation. they are considered as chemical preservative for whole blood sample, as it preserve the blood in a state similar to that find inside the body. There are different types of anticoagulants, as Ethylene Diamine Tetraacetic Acid (EDTA), Heparin, … Read more

Mean Corpuscular Values

Last Updated on December 7, 2018 by admin Three values are calculated mathematically and used mainly to measure the average size of the RBC, average hemoglobin weight and percent of hemoglobin per individual erythrocyte. The mean corpuscular values are: – Mean corpuscular volume (MCV), MCV is obtained by dividing the value of packed cells volume … Read more

Polycythemia

Last Updated on December 7, 2018 by admin Prof. Mahmoud Rushdi (Assiut University-Egypt) Definition Polycythemia is the increase of RBCs count above the normal upper limit specific for each animal species. There are two types of polycythemia, which are relative and absolute polycythemia. Relative polycythemia is defined as the relative increase of RBCs count due … Read more

The significance of blood film examination

Last Updated on December 7, 2018 by admin 1. Identification of different animal species. 2. Morphological examination of the erythrocytes and leucocytes. 3. Carrying out DLC. 4. Diagnosis of some types of leukaemia. 5. Diagnosis of blood parasites infections either intercellular or intracellular. 6. Diagnosis of bacterial diseases, during bacteraemia. 7. Prognosis of disease through … Read more

RBCs count

Last Updated on December 7, 2018 by admin There are two methods for counting RBCs; 1) Automated method through the electronic blood cells counter. 2) Manual method using the hemocytometer. Electronic counter is more accurate than the hemocytometer method. The electronic counter is expensive, needs periodical maintenance. Maintenance of blood counter is very important; failure … Read more

Glucose Tolerance Test

Last Updated on December 7, 2018 by admin Glucose tolerance test is one of the tests that used for diagnosis of diabetes mellitus. Diabetes mellitus is characterized by recurrent hyperglyaemia associated with glucosuria, polyuria and polydepsia. Their are two types of glucose tolerance test: Oral Glucose tolerance test and intravenous glucose tolerance test. For oral … Read more

Liver enzymes

Last Updated on December 7, 2018 by admin Liver enzymes are enzymes that found inside the hepatocytes (liver cells), which are released to the blood circulation when the liver cells are damaged during inflammation or infection by microorganism or parasite. There are different types of liver enzymes; the first is that group of enzymes that … Read more

White blood cells (WBCs) count

Last Updated on December 7, 2018 by admin White blood cells (WBCs) count There are six types of white blood cells circulates in the blood circulation, which are Neutrophils, lymphocytes, eosinophils, basophils, monocytes and band cells. White blood cells count is an integral part of complete blood count (CBC), and include two main components: 1- … Read more

Physiological Factors affecting the RBCs count

Last Updated on April 22, 2018 by admin The circulating red blood cells (RBCs) are produced by the bone marrow, stored in the spleen and circulate in the blood vessels. Life span of the erythrocytes The lifespan of the erythrocytes in human is varied from 90-120 days,. However, in animals the lifespan varies according to … Read more

Jaundice or Icterus Metabolism of bilirubin

Last Updated on October 22, 2017 by admin Jaundice or icterus is defined as the yellowish pigmentation of skin and mucous membranes especially conjunctiva and sclera, as a consequence of increased blood bilirubin level. There are two forms of bilirubin in the blood circulation, which are direct or conjugated and indirect or unconjugated bilirubin. The … Read more